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histopaque density  (Lonza)


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    Lonza histopaque density
    Histopaque Density, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histopaque+density/histopaque+density/pm40004010-300-8-12
    Average 90 stars, based on 1 article reviews
    histopaque density - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Human neutrophils are not activated by Zika virus but reduce the infection of susceptible cells
    Article Snippet: Cell viability was determined by Trypan blue exclusion assay and the neutrophil purification was confirmed by cytospin slides (Cytospin 4; Thermo Fisher Scientific) visualized by microscopy (LEICA AF6000 Modular System) ( ) and flow cytometry. .. Peripheral blood mononuclear cells (PBMCs) were isolated using Histopaque density 1.077 g/mL (Lonza). .. CD14 + cells were sorted with the MACS system (Miltenyi Biotec), according to the manufacturer instructions and seeded at 5×10 5 cells/mL in RPMI-1640 media with L-glutamine (Lonza) supplemented with 10% fetal bovine serum (FBS; Gibco), 25 μg/mL gentamicin (Gibco), 100 IU/μg/mL penicillin-streptomycin (Sigma-Aldrich), 12.5 ng/mL recombinant human GM-CSF (PeproTech), and 25 ng/mL recombinant human IL-4 (PeproTech).

    Article Title: Trypanosomatid Extracellular Vesicles as Potential Immunogens for Chagas Disease.
    Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated using Histopaque density 1.077 g/mL (Lonza, Basel, Switzerland). .. CD14+ monocytes were sorted using the MACS system (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions and seeded at 5 × 105 cells/mL in a 150 cm2 cell culture flask containing 20 mL of RPMI-1640 medium supplemented with 10% FBS, 25 μg/mL gentamicin, 12.5 ng/mL recombinant human GM-CSF (PeproTech, Rocky Hill, NJ, USA), and 25 ng/mL recombinant human IL-4 (PeproTech, Rocky Hill, NJ, USA).



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